Sterilizing the hospital
The effectiveness of the NTU VirusBom against various kinds of viruses has been confirmed repeat-edly. Now the academic world has turned to consider whether the compound could be used against highly infectious bacterial "superbugs," which pose even graver medical threats. Target No. 1 was NDM-1, an enzyme that makes bacteria resistant to nearly all antibiotics.
NDM-1 is resistant to many drugs and can replicate itself outside the chromosomes of many germs. By transmitting itself with Escherichia coli, Klebsiella pneumonia and other pathogens, it often proves deadly. First discovered at a hospital in New Delhi, it gradually spread, and outbreaks have been recorded in Pakistan, Hong Kong and even Japan.
The most frightening aspect of NDM-1 is that it renders almost all antibiotics ineffective. Currently, only a few last-line-of-defense antibiotics, such as tigecycline (Tygacil) and polymyxin E (Colistin), have been shown to have any effect on NDM-1-bearing bacteria in clinical trials. Patients are largely left to rely on their own immune systems to battle them.
In mid-September of 2010, a crew from the TVBS show Super Taste was mysteriously fired upon while on location in India. Two cameramen were seriously injured. One of them came down with NDM-1 while being treated in India. His return to Taiwan caused alarm in some quarters. Fortunately, he was symptomless and avoided a troublesome fever, pneumonia, or septicemia. After a short period in isolation, he recovered completely. His return didn't prompt an epidemic in Taiwan.
Yet the bacterial cultures taken from his body attracted a lot of attention in academia. Lai Hsin-chih, director of the Graduate Institute of Medical Biotechnology at Chang Gung University, had long been interested in controlling the spread of drug-resistant germs inside hospitals. With access to those cultures, he applied for a grant to research superbugs from the ROC Centers for Disease Control.
Lai points out that the warm and damp hospital environment is the site of frequent applications of disinfectants. Although the disinfectants kill most germs, a small number will prove resistant. NDM-1 isn't entirely unique. Other enzymes similarly confer resistance to many antibiotics on superbug strains of bacteria such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus.
These bacteria, widely present in hospitals, typically affect only those with compromised immune systems, such as terminal cancer patients, or those who have had major surgery, including organ transplants. The healthy usually are symptomless when they contract them. But these superbugs add a huge potential threat of post-operative complications for those who are ill.
The NTU VirusBom is not only effective against viruses. It even vanquishes nettlesome superbugs such as bacteria bearing the NDM-1 genetic trait. Researchers cultivate NDM-1 bacteria in petri dishes (left) and expose them to different concentrations of the NTU VirusBom (right). They've discovered that the superbugs cannot survive when exposed to the VirusBom in concentrations of 300 ppm.